Search and Browse
Download
Enter Data
| Solution Information | help | |
| Enzyme: | Inward rectifier potassium channel 2 | |
| inhibitor: | BDBM65855 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay overview: The purpose of this assay is for the confirmation dose responses for compounds that inhibit/block inward-rectifying potassium ion channel Kir2.1, that identified in the primary screen assay (PubChem AID 1672) and consequent specificity screens (PubChem AID 1843). It employs the same experimental conditions as presented in the primary screen assay, except in multiple concentrations. Compounds were tested in triplicates and their effects were evaluated by the calculated FluxOR fluorescence ratio. Protocol for the Kir2.1 project: 1. Cell culture: Cells are routinely cultured in DMEM/F12 medium, supplemented with 10% Fetal Bovine Serum (FBS), 50 IU/ml penicillin, 50ug/ml streptomycin, and 500ug/ml G418. 2. Cell plating: Cells were re-suspended in DMEM/F12 medium with 10% FBS at 300,000 cells/ml and added to the 384 well microtiter plates at 50 ul/well 3. Incubate overnight at 37#C and 5% CO2 4. Remove medium and add 25 ul/well of 1x FluxOR solution 5. Incubate 90 minut | |
| If you find an error in this entry please send us an E-mail | ||
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||